Journal: Molecular Medicine
Article Title: Androgens alleviate the depression-like phenotype in female mice by inhibiting AVPR1a in the hippocampal brain region
doi: 10.1186/s10020-025-01272-9
Figure Lengend Snippet: DHEA inhibited AVPR1a expression in HT22 cells via AR. A The expression of AVPR1a protein in HT22 cells treated with DHEA at different concentrations (0 µM, 10 µM and 20 µM) for different durations (24 h and 48 h) was detected by western blot. B , C The relative expression of AVPR1a protein ( B ) and mRNA ( C ) in HT22 cells treated with different concentrations (0 µM, 10 µM, and 20 µM) of DHEA for different periods (24 h and 48 h). D The expression of AVPR1a protein in DHEA-treated HT22 cells after antagonist pretreatment was detected by western blot. E , F The relative expression of AR protein ( E ) and mRNA ( F ) in DHEA-treated HT22 cells after antagonist pretreatment. G , H The relative expression of AVPR1a protein ( G ) and mRNA ( H ) in DHEA-treated HT22 cells after antagonist pretreatment. The data are shown as the mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc test was used for Fig. 6B-C and one-way ANOVA followed by Tukey’s post hoc test was used for Fig. 6E-F, G-H, and the experiment was repeated three times independently with similar results. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, ns indicates no significant difference
Article Snippet: The female mice were randomly divided into two groups and received treatment with DHEA (30 mg/kg/day, MCE, #HY-14650) or sesame oil (Beyotime, #ST2627) via abdominal subcutaneous injection.
Techniques: Expressing, Western Blot
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